First steps toward establishment ofTissue Engineering and Regenerative Medicine department in AvicennaResearch Institute (ARI) were taken in 2009, with long-term goal ofdeveloping new ways to repair, replace, reconstruct and regeneratedamaged or diseased tissues especially connective tissues and skindefects. Already, this department with integrating variousspecialists in the fields of stem cell, biomaterial, immunology andveterinary and also providing of required facilities andinstruments has been created a suitable substratum to developapplicable products. It is hoped that this group could be efficientin maturation and expansion of the therapeutically and medicallyservices.
- Cartilage Tissue Engineering
Despite over two decades of research on cartilage tissueengineering, very few products have moved from bench to bedside. Inthis project, with the gold objective of repair of articularcartilage defects using suitable scaffolds that have optimalstructural and mechanical properties, excellent biocompatibility,controlled degradation rate, and good handling characteristics, andalso greatly aided by recent advances in stem cell biology,feasibility of the developed cell/scaffold constructs by our groupfor repair of human articular cartilage have been evaluated usingcomprehensive designed in vitro and in vivo experiments. To developthe project, multidisciplinary specialists in tissue engineeringand regenerative medicine, biomaterial, veterinarian and immunologyhave successfully been contributed.
- Skin Tissue Engineering
Loss of skin which leads to chronic wounds seems to be a worldwidegrowing health problem. One of the most common reasons for majorskin loss is thermal trauma, where substantial areas of skin can bedamaged often without the possibility of skin tissue regeneration.These types of wounds pose hurdles to caregivers and are costly totreat. Today’s clinical gold standard for tackling this problem is“Autologous Grafting” which faces limitations such as painful donorsite, insufficient amount of graft, etc. Skin Tissue Engineering isan alternative strategy to overcome these difficulties. In thisproject, to find a proper skin tissue substitute, the efficacy ofthe various fabricated constructs through different combination ofnatural membranes, nano-fibers and stem cells would be evaluated invitro and in vivo using physical, mechanical, biodegradation andbiocompatibility experiments. The main goal is to establish moreefficient cell/scaffold constructs to repair of large skindefects.
- Isolation and culture of stem cells from different humanresources such as cord blood, bone marrow, menstrual blood anddental pulp.
- Generation of differentiated osteoblasts, adipocytes,chondrocytes, cardiomyocytes, hepatocytes and neuron-like cellsfrom different stem cells using optimized protocols.
- Genotypic and phenotypic characterization of undifferentiatedand differentiated stem cells by different molecular andcytochemical techniques such as RT-PCR, Real-Time PCR, Flowcytometry, immuno cytochemistry.
- Fabrication of 2D and 3D porous biodegradable polymeric,ceramic and composite scaffolds for Tissue Engineeringapplication
- Surface treatment and fictionalization of biomaterials
- Fabrication of nano-fibers and nano-structure membranes withhigh porosity and specific surface area (SSA)
- Three dimensional culture of stem cells and differentiatedcells in biodegradable and biocompatible scaffolds
- Breeding and housing of nude mice
- In vitro and in vivo evaluation of toxicity andbiocompatibility of drugs and synthetic scaffolds
- Possibility of short-term and long-term educational career inthe frame of workshop about stem cell culture and relatedtechniques.
DirectorAcademic StaffResearch Staff
List ofPublications :
- Mobini S, Javadpour J, Hosseinalipour M, Khavandi A, Rezaie HR,Ghazi- Khansari M. “ Synthesis and characterization of gelatin–nanohydroxyapatite composite scaffolds for bone tissue engineering “,Advances in Applied Ceramics. 2008, 107, 4‐8.
- Kazemnejad S, Allameh A, Soleimani M, Gharehbaghian A,Mohammadi Y, Amirizadeh N and Maryam Jazayeri. Biochemical andmolecular characterization of hepatocyte-like cells derived fromhuman bone marrow mesenchymal stem cells on a novelthree-dimensional biocompatible nanofibrous scaffold. Journal ofGastroenterology and hepatology. 2009, 24(2):278-287.
- Allameh A, Esmaeli S, Kazemnejad S, Soleimani M. Differentialexpression of glutathione S-transferases P1-1 and A1-1 at proteinand mRNA levels in hepatocytes derived from human bone marrowmesenchymal stem cells. Toxicology in vitro. 2009,23(4):674-679.
- Kazemnejad S. Hepatic tissue engineering using scaffold; stateof the art. Avicenna Journal of Medical Biotechnology. 2010,3:3-10.
- Ghamartaj Hossein, Manijeh Khanmohamadi, Neda Jarooghi, SomaiehKazemnejad. Evidence for an association of Wnt-independent cateninintracellular localization with ovarian apoptotic events in normaland PCO-induced rat ovary. J of PBS. 2011, 1(2): 1-10
- Kazemnejad Somaieh, Akhondi Mohammad-Mehdi, Soleimani Masoud,Zarnani Amir Hassan, Khanmohammadi Manijeh, Darzi Saeedeh.Characterization and chondrogenic differentiation of menstrualblood- derived stem cells on a nanofibrous scaffold. The Int J ofArtificial Organs. 2012, 35(1):55-66.
- Saeedeh Darzi, Amir Hassan Zarnani, Mahmood Jeddi-Tehrani, KEntezami Ebrahim Mirzadegan, Mohammad Mehdi Akhondi, Saeed Talebi,Manijeh Khanmohammadi, Somaieh Kazemnejad. Osteogenicdifferentiation of menstrual blood- versus bone marrow-derived stemcells in the presence of human platelet releasate. TissueEngineering. 2012, 18(15-16):1720-1728.
- Manijeh Khanmohammadi, Sayeh Khanjani, Mahsa Sani Bakhtiari,Amir Hassan Zarnani, Haleh Edalatkhah, Mohammad Mehdi Akhondi,Ebrahim Mirzadegan, Kourosh Kamali, Kamran Alimoghadam, SomaiehKazemnejad. Proliferation and chondrogenic differentiationpotential of menstrual blood- versus bone marrow-derived stem cellsin two-dimensional culture. Int J of Hematology. 2012, 95(5):484-493.
- Allameh Abdolamir, Kazemnejad Somaieh. Safety evaluation ofstem cells used for clinical cell therapy in chronic liverdiseases; with emphasize on biochemical markers. Clin Biochem.2012, 45:385-396.
- Shohreh Nikoo, Massoumeh Ebtekar, Mahmood Jeddi-Tehrani, AdelShervin, Mahmood Bozorgmehr, Somaieh Kazemnejad and Amir HassanZarnani. Effect of menstrual blood-derived stromal stem cells onproliferative capacity of peripheral blood mononuclear cells inallogeneic mixed lymphocyte reaction. 2012, 38(5):804-809.
- Mobini S, Solati‐Hashjin M, Hesaraki S, Gelinsky M.“Fabrication and characterization of regenerated silk/biolgasscomposites for bone tissue engineering“, Modares Journal of MedicalSciences: Pathobiology. 2012, 15(2), 47-60.
- Mobini S, Solati‐Hashjin M, Peirovi H, Samadikuchaksaraei A. “Synthesis and characterization of fiber reinforced polymer (FRP)scaffolds based on natural fibers and polymer”, Iranian Journal ofBiotechnology. 2012, 10(3), P184‐190.
- Kazemnejad S, Khanmohammadi M, Zarnani AH, Nikokar I, SaghariS. Role of wnt-signaling on proliferation of menstrual bloodderived stem cells. Journal of Stem Cells and RegenerativeMedicine. 2013, 9(1):8-12.
- Mobini S, Hoyer B, Lode A, Nosoudi N, Solati-Hashjin M,Samadikuchaksaraei A, Gelinsky M. " Fabrication andcharacterization of regenerated silk scaffolds reinforced withnatural silk fibers for bone tissue engineering ", Journal ofBiomedical Materials Research: Part A. 2013, 101(8):2392-2404.
- Mobini S, Solati‐Hashjin M, Peirovi H, Barati M, Gholipour M,Samadikuchaksaraei A. “Bioactivity and biocompatibility studies ona novel silk‐based scaffold for bone tissue engineering”, Journalof Medical and Biological Engineering. 2013, 33(2): 207‐214.
- Sayeh Khanjani, Manijeh Khanmohammadi,Saeed Talebi, HalehEdalatkhah,Amir Hassan Zarnani, Saman Eghtesad,Somaieh Kazemnejad.Efficient differentiation of menstrual blood derived stem cellsinto functional hepatocyte-like cells using various conversionprotocols. Journal of Tissue engineering and regenerative medicine.In press.
- Sayeh Khanjani, Manijeh Khanmohammadi, Amir-Hassan Zarnani,Mohammad-Mehdi Akhondi, Ali Ahani, Zahra Ghaempanah, SamanEghtesad, Somaieh Kazemnejad. Comparative evaluation ofdifferentiation potential of menstrual blood- versus bone marrow-derived stem cells into hepatocyte-like cells. PLOS ONE. Inpress.
- Delbandi AA, Mahmoudi M, Shervin A, Akbari E, Jeddi-Tehrani M,Sankian M, Kazemnejad S, Zarnani AH. Eutopic and ectopic stromalcells from patients with endometriosis exhibit differentialinvasive, adhesive, and proliferative behavior. Fertility andSterility. In press.
- Mobini S, Solati-Hashjin M, Peirovi H, Samadikuchaksaraei A. “Extraction and characterization of Bombyx Mori silk for medicalapplication”, Iranian Journal of Biomedical Engineering, 2013,5(2), 151-160.
- Somaieh Kazemnejad, Manijeh Khanmohammadi, Hamed Heidari-Vala,Mohammad Mehdi Akhondi. Advanced biology of adult stem cells. InPersian, 2013, Avicenna Research Institute. Tehran- Iran.
- Somaieh Kazemnejad, Amir Hasan Zarnani, Manijeh Khanmohammadi,Sahba Mobini. Chondrogenic differentiation of menstrual blood-derived stem cells on nanofibrous scaffolds. Methods MolecularBiology. 2013, 1058: 149–169.