Tissue Engineering and Regenerative Medicine (TERM) DepartmentPDFPrint

                             


  • About

  • Projects

  • Products/Services

  • Equipments

  • Members

  • Publications

First steps toward establishment of Tissue Engineering and Regenerative Medicine department in Avicenna Research Institute (ARI) were taken in 2009, with long-term goal of developing new ways to repair, replace, reconstruct and regenerate damaged or diseased tissues especially connective tissues and skin defects. Already, this department with integrating various specialists in the fields of stem cell, biomaterial, immunology and veterinary and also providing of required facilities and instruments has been created a suitable substratum to develop applicable products. It is hoped that this group could be efficient in maturation and expansion of the therapeutically and medically services.


Main Projects

  • Cartilage Tissue Engineering
    Despite over two decades of research on cartilage tissue engineering, very few products have moved from bench to bedside. In this project, with the gold objective of repair of articular cartilage defects using suitable scaffolds that have optimal structural and mechanical properties, excellent biocompatibility, controlled degradation rate, and good handling characteristics, and also greatly aided by recent advances in stem cell biology, feasibility of the developed cell/scaffold constructs by our group for repair of human articular cartilage have been evaluated using comprehensive designed in vitro and in vivo experiments. To develop the project, multidisciplinary specialists in tissue engineering and regenerative medicine, biomaterial, veterinarian and immunology have successfully been contributed.
  • Skin Tissue Engineering
    Loss of skin which leads to chronic wounds seems to be a worldwide growing health problem. One of the most common reasons for major skin loss is thermal trauma, where substantial areas of skin can be damaged often without the possibility of skin tissue regeneration. These types of wounds pose hurdles to caregivers and are costly to treat. Today’s clinical gold standard for tackling this problem is “Autologous Grafting” which faces limitations such as painful donor site, insufficient amount of graft, etc. Skin Tissue Engineering is an alternative strategy to overcome these difficulties. In this project, to find a proper skin tissue substitute, the efficacy of the various fabricated constructs through different combination of natural membranes, nano-fibers and stem cells would be evaluated in vitro and in vivo using physical, mechanical, biodegradation and biocompatibility experiments. The main goal is to establish more efficient cell/scaffold constructs to repair of large skin defects.


Products and services:

  • Isolation and culture of stem cells from different human resources such as cord blood, bone marrow, menstrual blood and dental pulp.
  • Generation of differentiated osteoblasts, adipocytes, chondrocytes, cardiomyocytes, hepatocytes and neuron-like cells from different stem cells using optimized protocols.
  • Genotypic and phenotypic characterization of undifferentiated and differentiated stem cells by different molecular and cytochemical techniques such as RT-PCR, Real-Time PCR, Flow cytometry, immuno cytochemistry.
  • Fabrication of 2D and 3D porous biodegradable polymeric, ceramic and composite scaffolds for Tissue Engineering application
  • Surface treatment and fictionalization of biomaterials
  • Fabrication of nano-fibers and nano-structure membranes with high porosity and specific surface area (SSA)
  • Three dimensional culture of stem cells and differentiated cells in biodegradable and biocompatible scaffolds
  • Breeding and housing of nude mice
  • In vitro and in vivo evaluation of toxicity and biocompatibility of drugs and synthetic scaffolds
  • Possibility of short-term and long-term educational career in the frame of workshop about stem cell culture and related techniques.


The Department of TERM unveiled locally-developed equipment that will let department to be more self-productive like:

Developed cell culture equipments
Electrospining Freeze dryer
Animal house for breading and housing


Technicians:
  • Morteza Farab, B.Sc
  • Zahra Ghaempanah, B.Sc
  • Farhad Hosseini, B.Sc


List of Publications :

Peer-reviewd

  • Mobini S, Javadpour J, Hosseinalipour M, Khavandi A, Rezaie HR, Ghazi- Khansari M. “ Synthesis and characterization of gelatin–nano hydroxyapatite composite scaffolds for bone tissue engineering “, Advances in Applied Ceramics. 2008, 107, 4‐8.
  • Kazemnejad S, Allameh A, Soleimani M, Gharehbaghian A, Mohammadi Y, Amirizadeh N and Maryam Jazayeri. Biochemical and molecular characterization of hepatocyte-like cells derived from human bone marrow mesenchymal stem cells on a novel three-dimensional biocompatible nanofibrous scaffold. Journal of Gastroenterology and hepatology. 2009, 24(2):278-287.
  • Allameh A, Esmaeli S, Kazemnejad S, Soleimani M. Differential expression of glutathione S-transferases P1-1 and A1-1 at protein and mRNA levels in hepatocytes derived from human bone marrow mesenchymal stem cells. Toxicology in vitro. 2009, 23(4):674-679.
  • Kazemnejad S. Hepatic tissue engineering using scaffold; state of the art. Avicenna Journal of Medical Biotechnology. 2010, 3:3-10.
  • Ghamartaj Hossein, Manijeh Khanmohamadi, Neda Jarooghi, Somaieh Kazemnejad. Evidence for an association of Wnt-independent catenin intracellular localization with ovarian apoptotic events in normal and PCO-induced rat ovary. J of PBS. 2011, 1(2): 1-10
  • Kazemnejad Somaieh, Akhondi Mohammad-Mehdi, Soleimani Masoud, Zarnani Amir Hassan, Khanmohammadi Manijeh, Darzi Saeedeh. Characterization and chondrogenic differentiation of menstrual blood- derived stem cells on a nanofibrous scaffold. The Int J of Artificial Organs. 2012, 35(1):55-66.
  • Saeedeh Darzi, Amir Hassan Zarnani, Mahmood Jeddi-Tehrani, K Entezami Ebrahim Mirzadegan, Mohammad Mehdi Akhondi, Saeed Talebi, Manijeh Khanmohammadi, Somaieh Kazemnejad. Osteogenic differentiation of menstrual blood- versus bone marrow-derived stem cells in the presence of human platelet releasate. Tissue Engineering. 2012, 18(15-16):1720-1728.
  • Manijeh Khanmohammadi, Sayeh Khanjani, Mahsa Sani Bakhtiari, Amir Hassan Zarnani, Haleh Edalatkhah, Mohammad Mehdi Akhondi, Ebrahim Mirzadegan, Kourosh Kamali, Kamran Alimoghadam, Somaieh Kazemnejad. Proliferation and chondrogenic differentiation potential of menstrual blood- versus bone marrow-derived stem cells in two-dimensional culture. Int J of Hematology. 2012, 95(5): 484-493.
  • Allameh Abdolamir, Kazemnejad Somaieh. Safety evaluation of stem cells used for clinical cell therapy in chronic liver diseases; with emphasize on biochemical markers. Clin Biochem. 2012, 45:385-396.
  • Shohreh Nikoo, Massoumeh Ebtekar, Mahmood Jeddi-Tehrani, Adel Shervin, Mahmood Bozorgmehr, Somaieh Kazemnejad and Amir Hassan Zarnani. Effect of menstrual blood-derived stromal stem cells on proliferative capacity of peripheral blood mononuclear cells in allogeneic mixed lymphocyte reaction. 2012, 38(5):804-809.
  • Mobini S, Solati‐Hashjin M, Hesaraki S, Gelinsky M. “Fabrication and characterization of regenerated silk/biolgass composites for bone tissue engineering“, Modares Journal of Medical Sciences: Pathobiology. 2012, 15(2), 47-60.
  • Mobini S, Solati‐Hashjin M, Peirovi H, Samadikuchaksaraei A. “ Synthesis and characterization of fiber reinforced polymer (FRP) scaffolds based on natural fibers and polymer”, Iranian Journal of Biotechnology. 2012, 10(3), P184‐190.
  • Kazemnejad S, Khanmohammadi M, Zarnani AH, Nikokar I, Saghari S. Role of wnt-signaling on proliferation of menstrual blood derived stem cells. Journal of Stem Cells and Regenerative Medicine. 2013, 9(1):8-12.
  • Mobini S, Hoyer B, Lode A, Nosoudi N, Solati-Hashjin M, Samadikuchaksaraei A, Gelinsky M. " Fabrication and characterization of regenerated silk scaffolds reinforced with natural silk fibers for bone tissue engineering ", Journal of Biomedical Materials Research: Part A. 2013, 101(8):2392-2404.
  • Mobini S, Solati‐Hashjin M, Peirovi H, Barati M, Gholipour M, Samadikuchaksaraei A. “Bioactivity and biocompatibility studies on a novel silk‐based scaffold for bone tissue engineering”, Journal of Medical and Biological Engineering. 2013, 33(2): 207‐214.
  • Sayeh Khanjani, Manijeh Khanmohammadi,Saeed Talebi, Haleh Edalatkhah,Amir Hassan Zarnani, Saman Eghtesad,Somaieh Kazemnejad. Efficient differentiation of menstrual blood derived stem cells into functional hepatocyte-like cells using various conversion protocols. Journal of Tissue engineering and regenerative medicine. In press.
  • Sayeh Khanjani, Manijeh Khanmohammadi, Amir-Hassan Zarnani, Mohammad-Mehdi Akhondi, Ali Ahani, Zahra Ghaempanah, Saman Eghtesad, Somaieh Kazemnejad. Comparative evaluation of differentiation potential of menstrual blood- versus bone marrow- derived stem cells into hepatocyte-like cells. PLOS ONE. In press.
  • Delbandi AA, Mahmoudi M, Shervin A, Akbari E, Jeddi-Tehrani M, Sankian M, Kazemnejad S, Zarnani AH. Eutopic and ectopic stromal cells from patients with endometriosis exhibit differential invasive, adhesive, and proliferative behavior. Fertility and Sterility. In press.
  • Mobini S, Solati-Hashjin M, Peirovi H, Samadikuchaksaraei A. “ Extraction and characterization of Bombyx Mori silk for medical application”, Iranian Journal of Biomedical Engineering, 2013, 5(2), 151-160.

    • Book/Book chapter

      • Somaieh Kazemnejad, Manijeh Khanmohammadi, Hamed Heidari-Vala, Mohammad Mehdi Akhondi. Advanced biology of adult stem cells. In Persian, 2013, Avicenna Research Institute. Tehran- Iran.
      • Somaieh Kazemnejad, Amir Hasan Zarnani, Manijeh Khanmohammadi, Sahba Mobini. Chondrogenic differentiation of menstrual blood- derived stem cells on nanofibrous scaffolds. Methods Molecular Biology. 2013, 1058: 149–169.



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Sample Chapter

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Last Updated on Sunday, 18 May 2014 08:11